GB/T 35803-2018 Translated English of Chinese Standard. (GBT 35803-2018, GB/T35803-2018, GBT35803-2018): Determination of prohibited urocanic acid and its ethyl ester in cosmetics - High performance liquid chromatographyThis Standard specifies determination method of high performance liquid chromatography for prohibited urocanic acid and its ethyl ester in cosmetics. This Standard is applicable to determination of urocanic acid and its ethyl ester in cosmetics such as creams, liquids, lipsticks, loose powders, shampoos. The detection limit of this Standard for urocanic acid and its ethyl ester is 2 mg/kg, and the limit of quantification is 5 mg/kg. |
Contents
| 3 | |
Introduction | 4 |
Scope | 5 |
Apparatus and equipment | 6 |
Analysis steps | 7 |
Result calculation | 8 |
Detection limit and limit of quantification | 9 |
Annex A informative Chinese name English name CAS number molecular formula relative molecular mass structural formula of urocanic acid and | 10 |
ethyl ester Error Bookmark not defined Annex C informative Test of confirmation | 13 |
Common terms and phrases
0.005mol/L tetrabutylammonium bromide 10mL of methanol 10mL plugged plastic acid ethyl ester Add into 9mL aqueous methanol solution blow tube 5.8 CAS number Chinese name chromatogram of urocanic conditions in 6.2 conditions The reference content of urocanic detection limit determination conditions English name ester in cosmetics ester mixed standard ester standard product ethyl ester mixed ethyl ester standard extraction for 20min Figure B.1 formula of urocanic GB/T graduated nitrogen blow high performance liquid ion pair m/z limit of quantification liquid chromatography-mass spectrometry Mass spectrometry membrane for high methanol 4.1 methanol solution 4.7 mg/kg microporous membrane milligrams mixed standard stock molecular formula nitrogen blow tube performance liquid chromatogram performance liquid chromatography plastic centrifuge tube plugged plastic centrifuge prohibited substances reference conditions relative molecular mass sample solution set volume specified specimen spectrum of urocanic standard stock solutions structural formula Table A.1 Tetrahydrofuran ultraviolet absorption spectrum urocanic acid ethyl Urocanic acid standard volume to scale